Confirm manual entries with ENTER. Exit the parameter level at any time by repeatedly pressing the. “Parameter” key. BioPhotometer – Short instructions. Get Eppendorf BioPhotometer Manual. Get all Eppendorf manuals!. Get Eppendorf BioPhotometer plus Instruction Manual. Get all Eppendorf manuals!.
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Eppendorf BioPhotometer User Manual
Take advantage of the two-in-one cuvette, decide based on your sample. UV-vis is your standard? The compact instrument clearly processes data, records at fixed wavelengths and makes evaluating results fast and simple.
For specific methods, additional measurements are recorded and displayed as a graph, making impurities easier and quicker to identify. The Eppendorf BioPhotometer D30 is bikphotometer 3rd generation of Eppendorf spectrophotometers to become an established standard in the life sciences.
Routine pre-defined wavelengths biophootometer easily selectable, allowing for quick and simple programming. Users are guided through every step of the assay with short, simple on-screen instructions. Raw and processed data are clearly presented on the large color screen and can be exported directly from the instrument in a variety of common formats.
Nucleic acid purification is a major application in molecular laboratories.
Purity and homogeneity of the sample are important considerations for subsequent applications. In practice, nucleic acids are purified with the help of commercially available kits which allow for the separation of most cellular components. However, the presence of proteins or other organic manyal in the eluate may not be entirely eliminated. When purifying nucleic acid without a kit, additional contamination risks are posed by the chemicals used in the process, e.
To ensure minimum contamination of the sample, it is essential to verify its purity by photometric measurements.
The following values represent a pure DNA sample: In general, the purity of a DNA solution is easily defined using purity ratios. It is manuzl that reduced purity may be attributed to the fact that the sample is solubilized in water instead of in buffer.
Cell Protein DNA Core Facilities
Since water and buffer have different properties, the absorbance of the sample being measured will be affected by this disparity. In addition, all important raw data pertaining to the DNA sample are displayed in table format.
Thus, the two display formats complement each other perfectly to allow for an evaluation of the quality of a DNA sample. The ideal Eppendorf product fulfilling the PhysioCare concept provides a comprehensive approach for the user. Starting with an ergonomic design of the product itself e. Further information about the Eppendorf PhysioCare Concept: Forgot password or username? Similar dimensions as classic cuvettes.
Easy closing for easy handling. Up to 3 mL filling volume. Easy and intuitive sample processing. Product Information The Eppendorf BioPhotometer D30 is the 3rd generation of Eppendorf spectrophotometers to become an established standard in the life sciences.
Results In addition, all important raw data pertaining to the DNA sample are displayed in table format. Operating manual — BioPhotometer D Application Note — Detection of contamination in DNA and protein samples by photometric measurements. Poster — Nucleic Acid Photometry. White Paper — Better safe than sorry.
Eppendorf macro Vis Cuvetteplastic cuvette for measurements in the Vis range, max. Eppendorf semi-micro Vis Cuvetteplastic cuvette for measurements in the Vis range, max.
RNA and valuable samples Deep-lying optical window prevents scratches Tapered cuvette base for optimal filling, frosted grip for labeling Ideal for use in the Nanual BioPhotometer and Eppendorf BioSpectrometer, adapters available for use with other common spectrophotometers Routine pack for convenient access to each UVette and safe storage in a reclosable box.
UVettes in routine packs are ideal for routine applications. These packs allow a safe storage of the UVettes in a reclosable box, while offering convenient access to each cuvette for easy filling.
Microvolume measuring cell for measuring 1. Service product portfolio may vary according to country. Checking condition of delivery Set-up and initial operation Instruction of biophoto,eter Self test where applicable IQ Certificate.
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Contact us Distributors Technical Service. Our website biophoometer not support your outdated browser version. Please update to a current version, e. For connecting to a network printer or emailing data outputs directly off instrument.